Journal
CURRENT OPINION IN BIOTECHNOLOGY
Volume 21, Issue 4, Pages 517-523Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2010.08.002
Keywords
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Funding
- Engineering and Physical Sciences Research Council [EP/I005471/1, EP/H049479/1] Funding Source: researchfish
- EPSRC [EP/I005471/1, EP/H049479/1] Funding Source: UKRI
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Microfluidic systems miniaturise biological experimentation leading to reduced sample volume, analysis time and cost. Recent innovations have allowed the application of -omics approaches on the microfluidic scale. It is now possible to perform 1.5 million PCR reactions simultaneously, obtain transcriptomic data from as little as 150 cells (as few as 2 transcripts per gene of interest) and perform mass-spectrometric analyses online. For synthetic biology, unit operations have been developed that allow de novo construction of synthetic systems from oligonucleotide synthesis through to high-throughput, high efficiency electroporation of single cells or encapsulation into abiotic chassis enabling the processing of thousands of synthetic organisms per hour. Future directions include a push towards integrating more processes into a single device and replacing off-chip analyses where possible.
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