Journal
CURRENT NANOSCIENCE
Volume 10, Issue 5, Pages 730-735Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1573413710666140116213526
Keywords
Cyclic voltammetry; functionalization; alpha-glucosidase; immobilization
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Funding
- University Malaysia Perlis
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alpha-glucosidase (AG) enzyme was immobilized onto amine functionalized multi-walled carbon nanotubes (MWCNTs-NH2) by treatment with cross linkers, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) via amide bonding. The immobilized AG was characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). The performance of the electrode was evaluated by Spectrophotometric method and cyclic voltammetric (CV) method using p-nitrophenyl-alpha-D-glucopyranoside (PNPG) as a substrate. The immobilized AG enzyme retains about 84.16% activity of its initial response even after 30 days when stored at 4 degrees C.
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