Journal
CURRENT MICROBIOLOGY
Volume 68, Issue 5, Pages 587-593Publisher
SPRINGER
DOI: 10.1007/s00284-013-0513-4
Keywords
-
Categories
Funding
- national natural science foundation of China [21077090, 21137003]
- SRF for ROCS, SEM [J20120548, ZEM Z201010001]
Ask authors/readers for more resources
The culture-dependent method and a degenerate primer-based culture-independent method were combined in an effort to identify N-acyl homoserine lactone (AHL) producers in rhizosphere of wetland plants, Salix babylonica (willow) and Phragmites australis (reed). Overall, eight potential AHL-producing genera were found, which were Aeromonas, Pseudomonas, Polymorphum, Agrobacterium, Rhizobium, Sinorhizobium, Ensifer, and Pectobacterium. Thin layer chromatograph assay revealed various AHL profiles from cultivable AHL-producers. The degenerate primer pair RAHL352F and RAHL461R was found to cover AHL synthetase genes from families Rhizobiaceae and Rhodobacteraceae. Little overlap was found in taxa of potential AHL-producers obtained by the two methods, indicating that they were well complement to each other. This is the first survey for AHL-producers that employed combined culture-dependent and -independent methods.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available