Journal
CURRENT DRUG TARGETS
Volume 12, Issue 11, Pages 1547-1559Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/138945011798109527
Keywords
Antibacterials; peptides; screening approaches; genetic engineering; chemical screening; combinatorial biosynthesis; mutasynthesis; precursor-directed biosynthesis
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Funding
- Deutsche Forschungsgemeinschaft (DFG)
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After decades of neglect in industrial research the comeback of natural products is due since improved screening approaches are at disposal, yielding a multitude of new compounds from natural sources. Besides traditional compound libraries peptides are characterized by an enormous structural complexity, thus increasing the chance of finding a hit in a screening. Emphasizing antibacterial compounds structural complexity is a prerequisite for their success. This review focuses on the screening approaches employed for the discovery of mostly antibacterial, non-ribosomal peptides derived from natural sources. Traditional screening methodologies as well as genetic approaches are discussed in this context. Utilizing genetic engineering methods e.g., precursor-directed biosynthesis, mutasynthesis, combinatorial biosynthesis, as well as chemoenzymatics to achieve greater structural diversity is thoroughly discussed and exemplified by recent discoveries.
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