Journal
CURRENT BIOLOGY
Volume 23, Issue 24, Pages 2534-2539Publisher
CELL PRESS
DOI: 10.1016/j.cub.2013.10.052
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Funding
- Wellcome Trust
- MRC
- CRUK
- Austrian Academy of Sciences
- Cancer Research UK [13031] Funding Source: researchfish
- Medical Research Council [G0701161] Funding Source: researchfish
- MRC [G0701161] Funding Source: UKRI
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Since the dissolution of sister chromatid cohesion by separase and cyclin B destruction is irreversible, it is essential to delay both until all chromosomes have bioriented on the mitotic spindle. Kinetochores that are not correctly attached to the spindle generate the mitotic checkpoint complex (MCC), which inhibits the anaphase-promoting complex/cyclosome (APC/C) and blocks anaphase onset. This process is known as the spindle assembly checkpoint (SAC) [1]. The SAC is especially important in meiosis I, where bivalents consisting of homologous chromosomes held together by chiasmata biorient. Since the first meiotic division is unaffected by rare achiasmatic chromosomes or misaligned bivalents [2-7], it is thought that several tensionless kinetochores are required to produce sufficient MCC for APC/C inhibition. Consistent with this, univalents lacking chiasmata elicit a SAC-mediated arrest in M/h1(-/-) oocytes. In contrast, chromatids generated by TEV protease-induced cohesin cleavage in Rec8(TEV/TEV) oocytes merely delay APC/C activation. Since the arrest of M/h1(-/-) Rec8(TEV/TEV)oocytes is alleviated by TEV protease, even when targeted to kinetochores, we conclude that their SAC depends on cohesin as well as dedicated kinetochore proteins. This has important implications for aging oocytes [8, 9], where cohesin deterioration will induce sister kinetochore biorientation and compromise MCC production, leading to chromosome missegregation and aneuploid fetuses.
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