Journal
CURRENT BIOLOGY
Volume 20, Issue 20, Pages 1787-1798Publisher
CELL PRESS
DOI: 10.1016/j.cub.2010.09.006
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Funding
- Fundacao para a Ciencia e a Tecnologia of Portugal
- Japanese Society for the Promotion of Science (JSPS)
- Japan's Ministry of Education, Culture, Sports, Science and Technology
- Netherlands Genomics Initiative
- Medical Research Council
- Wellcome Trust
- Medical Research Council [G0501615] Funding Source: researchfish
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Background: Developmental abnormalities observed in Cornelia de Lange syndrome have been genetically linked to mutations in the cohesin machinery. These and other recent experimental findings have led to the suggestion that cohesin, in addition to its canonical function of mediating sister chromatid cohesion, might also be involved in regulating gene expression. Results: We report that cleavage of cohesin's kleisin subunit in postmitotic Drosophila salivary glands induces major changes in the transcript levels of many genes. Kinetic analyses of changes in transcript levels upon cohesin cleavage reveal that a subset of genes responds to cohesin cleavage within a few hours. In addition, cohesin binds to most of these loci, suggesting that cohesin is directly regulating their expression. Among these genes are several that are regulated by the steroid hormone ecdysone. Cytological visualization of transcription at selected ecdysone-responsive genes reveals that puffing at Eip74EF ceases within an hour or two of cohesin cleavage, long before any decline in ecdysone receptor could be detected at this locus. Conclusion: We conclude that cohesin regulates expression of a distinct set of genes, including those mediating the ecdysone response.
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