Journal
JOURNAL OF NEUROIMMUNOLOGY
Volume 286, Issue -, Pages 42-47Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneuroim.2015.06.013
Keywords
Acute ischemic stroke; Brain derived neurotrophic factor; FoxP3; Regulatory T cells; Flow cytometry; Protective immunity; Neuroprotection; Stroke outcome
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Funding
- Royal Brisbane and Women's Hospital Foundation
- National Heart Foundation of Australia
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The aim of this study was to measure the levels of circulating BDNF and the frequency of BDNF-producing T cells after acute ischaemic stroke. Serum BDNF levels were measured by ELISA. Flow cytometry was used to enumerate peripheral blood leukocytes that were labelled with antibodies against markers of T cells, T regulatory cells (Tregs), and intracellular BDNF. There was a slight increase in serum BDNF levels after stroke. There was no overall difference between stroke patients and controls in the frequency of CD4(+) and CD8(+) BDNF+ cells, although a subgroup of stroke patients showed high frequencies of these cells. However, there was an increase in the percentage of BDNF+ Treg cells in the CD4(+) population in stroke patients compared to controls. Patients with high percentages of CD4(+) BDNF+ Treg cells had a better outcome at 6 months than those with lower levels. These groups did not differ in age, gender or initial stroke severity. Enhancement of BDNF production after stroke could be a useful means of improving neuroprotection and recovery after stroke. (C) 2015 Elsevier B.V. All rights reserved.
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