Journal
CURRENT ANALYTICAL CHEMISTRY
Volume 10, Issue 2, Pages 280-287Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/15734110113099990003
Keywords
Efavirenz; DLLME; plasma; HPLC; HIV; UV detection.
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Funding
- Islamic Azad University of Khorramabad
- Lorestan University of Medical Sciences
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Dispersive liquid-liquid microextraction (DLLME) coupled with high-performance liquid chromatography (HPLC) equipped with UV detector was used for the extraction and determination of efavirenz in plasma sample. Acetonitrile and chloroform were used as disperser and extraction solvents, respectively. Several parameters, including extraction solvent, the type of dispersion solvent, volume of extraction and dispersion solvents, pH, ionic strength of the media, as well as centrifuging time were optimized. Samples were injected on a reversed-phase C-18 analytical column. The mobile phase consisted of 20 mM phosphate buffer (pH-3.0) and acetonitrile (40: 60, v/v). The calibration curve of the proposed method was linear in the range of 0.1-100 mu g/L. The relative standard deviations (RSD, %) were 3.4-7.5% (n-6) and the limit of detection (LOD) was 0.01 mu g/L. The proposed method was applied to the analysis of plasma sample and spiked recoveries in the range of 98.5-102.5% were obtained. The obtained results show that DLLME is a very simple, rapid, sensitive, and efficient analytical method for the determination of efavirenz in plasma.
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