Journal
CURRENT ALZHEIMER RESEARCH
Volume 8, Issue 7, Pages 741-752Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/156720511797633223
Keywords
Anesthesia; Alzheimer's disease; isoflurane; apoptosis; beta-Amyloid protein; dual effects; cytosolic calcium
Categories
Funding
- National Institutes of Health [K08NS048140, R21AG029856, R01 GM088801, R01GM079360]
- American Geriatrics Society
- Alzheimer's Association
- China National Science Foundation [NSF30928026]
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beta-amyloid protein (A beta)-induced neurotoxicity is the main component of Alzheimer's disease (AD) neuropathogenesis. Inhalation anesthetics have long been considered to protect against neurotoxicity. However, recent research studies have suggested that the inhalation anesthetic isoflurane may promote neurotoxicity by inducing apoptosis and increasing A beta levels. We therefore set out to determine whether isoflurane can induce dose-and time-dependent dual effects on A beta-induced apoptosis: protection versus promotion. H4 human neuroglioma cells, primary neurons from nave mice, and nave mice were treated with A beta and/or isoflurane, and levels of caspase-3 cleavage (activation), apoptosis, Bcl-2, Bax, and cytosolic calcium were determined. Here we show for the first time that the treatment with 2% isoflurane for six hours or 30 minutes potentiated, whereas the treatment with 0.5% isoflurane for six hours or 30 minutes attenuated, the A beta-induced caspase-3 activation and apoptosis in vitro. Moreover, anesthesia with 1.4% isoflurane for two hours potentiated, whereas the anesthesia with 0.7% isoflurane for 30 minutes attenuated, the A beta-induced caspase-3 activation in vivo. The high concentration isoflurane potentiated the A beta-induced reduction in Bcl-2/Bax ratio and caused a robust elevation of cytosolic calcium levels. The low concentration isoflurane attenuated the A beta-induced reduction in Bcl-2/Bax ratio and caused only a mild elevation of cytosolic calcium levels. These results suggest that isoflurane may have dual effects (protection or promotion) on A beta-induced toxicity, which potentially act through the Bcl-2 family proteins and cytosolic calcium. These findings would lead to more systematic studies to determine the potential dual effects of anesthetics on AD-associated neurotoxicity.
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