Journal
CRYOBIOLOGY
Volume 68, Issue 3, Pages 419-430Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2014.03.005
Keywords
Mouse oocytes and embryos; Ultra-rapid warming; Laser; Dehydration; Recrystallization of ice; Clyo Jig; Functional survival
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Funding
- NIH [R01-OD011201]
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Vitrification is the most sought after route to the cryopreservation of animal embryos and oocytes and other cells of medical, genetic, and agricultural importance. Current thinking is that successful vitrification requires that cells be suspended in and permeated by high concentrations of protective solutes and that they be cooled at very high rates to below -100 degrees C. We report here that neither of these beliefs holds for mouse oocytes. Rather, we find that if mouse oocytes are suspended in media that produce considerable osmotic dehydration before vitrification and are subsequently warmed at ultra high rates (10,000,000 degrees C/min) achieved by a laser pulse, nearly 100% will survive even when cooled rather slowly and when the concentration of solutes in the medium is only 1/3rd of standard. (C) 2014 Elsevier Inc. All rights reserved.
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