Journal
CRYOBIOLOGY
Volume 64, Issue 3, Pages 211-214Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2012.01.010
Keywords
Mouse; B6D2F1; C57BL/6; Sperm; Freeze-drying; Tris-EDTA buffer; Long term preservation; ICSI
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- JSPS
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Many genetically engineered mice strains have been generated worldwide and sperm preservation is a valuable method for storing these strains as genetic resources. Freeze-drying is a useful sperm preservation method because it requires neither liquid nitrogen nor dry ice for preservation and transportation. We report here successful long-term preservation at 4 degrees C of mouse spermatozoa freeze-dried using a simple buffer solution (10 mM Iris, 1 mM EDTA, pH 8.0). Offspring with fertility were obtained from oocytes fertilized with freeze-dried spermatozoa from C57BL/6 and B6D2F1 mouse strains stored at 4 degrees C for 3 years. This freeze-drying method is a safe and economical tool for the biobanking of valuable mouse strains. (C) 2012 Elsevier Inc. All rights reserved.
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