Journal
CRYOBIOLOGY
Volume 61, Issue 2, Pages 231-233Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2010.06.011
Keywords
Inexpensive temperature measurement; High rate; Warming; Cooling; Type T thermocouple; USB oscilloscope; Cryotop
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Funding
- US National Institutes of Health [R01-RR 18470]
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We have developed a simple, inexpensive system (<$300 US) for measuring cooling and warming rates of small (similar to 0.1 mu l) aqueous Samples at rates as high as 10(5) degrees C/min. The measurement system itself, can track rates approaching one million degrees C/min. For temperature sensing, a Type T thermocouple with 50 mu m wire was used. The thermocouple output voltage was read with an inexpensive USB based digital oscilloscope interfaced to a laptop computer, and the raw data were processed with MS Excel. (C) 2010 Elsevier Inc. All rights reserved.
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