Journal
CROP PROTECTION
Volume 61, Issue -, Pages 1-10Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cropro.2014.02.012
Keywords
Sheath blight disease; Streptomyces philanthi; Antifungal compound; Rhizoctonia solani; Biological control
Categories
Funding
- Office of the Higher Education Commission under the CHE-PhD Scholarship Program
- Higher Research Promotion and National Research University Project of Thailand
- Graduate School of Prince of Songkla University
- National Biological Control Research Center Southern Regional Center [RM-1-138]
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Streptomyces philanthi RM-1-138, isolated from the rhizosphere soil of chili pepper in southern Thailand, was investigated for its antagonistic activity against phytopathogenic fungi. In dual culture on glucose yeast-malt extract (GYM) agar plates, this strain suppressed the mycelial growth of all seven plant pathogenic fungi tested (Rhizoctonia solani PTRRC-9, Pyricularia grisea PTRRC-18, Colletotri chum gloeosporioides NBCRSR-3, Colletotrichum capcisi NBCRSR-15, Ganoderma boninense NBCRSR-26, Fusarium fujikuroi PTRRC-16 and Bipolaris oryzae PTRRC-36) with an 82.2-89.2% inhibition and that was most pronounced on R. solani PTRRC-9. Heat treatment of the culture filtrate from growing R. solani PTRRC-9 at 40 degrees C, 60 degrees C, 80 degrees C, and 100 degrees C for 30 min and 121 degrees C for 15 min had no negative effect on the inhibitory activity against R. solani PTRRC-9 tested on both solid and liquid culture. The effective dose (>80% inhibition) of culture filtrate in liquid culture was at 5.0% (v/v) while it was at 10% (v/v) on the solid medium. This effectiveness was similar to those of the four chemical fungicides tested. The effect of S. philanthi RM-1-138 against R. solani PTRRC-9 was investigated using SEM and TEM. The compounds produced by S. philanthi RM-1-138 induced alterations to the cell-wall structure of R. solani PTRRC-9, that resulted in the loss of cytoplasm materials by partial lysis. The greenhouse experiment revealed that using either the culture filtrate or the autoclaved culture filtrate from S. philanthi RM-1-138 effectively suppressed rice sheath blight disease by up to 65.6 and 60.8%, respectively. (C) 2014 Elsevier Ltd. All rights reserved.
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