Journal
CROP PROTECTION
Volume 52, Issue -, Pages 141-150Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cropro.2013.05.023
Keywords
Resistance field; Benzimidazole; Sterol demethylation inhibitors (DMI); AFLP; Beta-tubulin; Aggressiveness
Categories
Funding
- Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentarias [INIA RTA04-127, INIA RTA2007-07]
- FEDER funds
- Programa Personal Tecnico de Apoyo, Ministerio de Educacion y Ciencia [PTA-2003-01-01001]
- Contratos Doctores Sistema INIA-CCAA [37, DR07-0161]
Ask authors/readers for more resources
Fungicide resistance in Phaeoacremonium aleophilum, one of the most frequent fungal pathogens associated with grapevine trunk diseases, was investigated and found to exist in some isolates of the pathogen against a commercial formulation, Escudo (R). The effect of this compound and its two active substances, carbendazim and flusilazole, was first evaluated on the mycelial growth of P. aleophilum. Escudo (R)-resistant isolates were estimated at a frequency of 24% in Spanish vineyards. Then, the two active substances were used individually to test their effect on mycelia growth of twelve single-spore cultures originating from six Escudo (R)-resistant isolates. Flusilazole (DMI-triazole) did not inhibit mycelia growth of any single-spore cultures of P. aleophilum. Carbendazim (benzimidazole) used alone allowed the growth of the same single-spore cultures that were also resistant to Escudo (R). AFLP characterization of sensitive and resistant single-spore cultures showed genetic diversity within P. aleophilum isolates but no AFLP markers were associated with resistance. New primers set (L2/R1) were designed to partially amplify the exon 6 of the beta-tubulin gene of P. aleophilum. Two different point mutations resulted in glycine (GGC) or lysine (AAA) replacing the glutamic acid (GAG) at codon 198 of the beta-tubulin gene in some of the resistant single-spore cultures studied. Resistant single-spore cultures of P. aleophilum were shown to have different aggressiveness levels as sensitive single-spore cultures by inoculation of wood segments of Vitis vinifera in the presence and absence of fungicide. (C) 2013 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available