Journal
JOURNAL OF NATURAL MEDICINES
Volume 70, Issue 2, Pages 296-299Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s11418-015-0949-x
Keywords
Immunoassay; Miroestrol; Pueraria candollei; Mannich reaction; Immunogen
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Funding
- Royal Golden Jubilee PhD program by Thailand Research Fund organization [PHD/0050/2556]
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Immunogen quality is one important factor that contributes to desirable antibody characteristics. Highly specific antibodies against miroestrol can be used to develop a quality control immunoassay for Pueraria candollei products. In this study, we investigated how various immunogen preparations affect antibody properties. The results show that immunogen prepared using the Mannich reaction provides antibodies with higher specificity and sensitivity against miroestrol than immunogen prepared with the periodate reaction. The results suggest the Mannich reaction maintains the original structure of miroestrol and generates useful antibodies for developing immunoassays.
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