Separation and Quantification of 1,4-benzodiazepines: HPLC versus CZE

The goal of the present study was to separate a set of benzodiazepines, namely bromazepam, oxazepam, nitrazepam, chlordiazepoxide, flunitrazepam, lormetazepam and diazepam by analytical scale HPLC and CZE. The both methods for separation of these seven compounds from the 1,4-benzodiazepine group were optimized and compared. LODs and LOQs were determined under the optimized conditions in the both methods. The corresponding LOD and LOQ values are approximately three orders of magnitude lower in HPLC than in CZE. As expected, elution order was found to be different for the both techniques. As a result of a critical collation of all the parameters considered, RP-HPLC was found to be more suitable for determination of the set of benzodiazepines. A real sample analysis was performed under optimized conditions to demonstrate applicability of the proposed analytical methods. (doi: 10.5562/cca1738)