Journal
CRITICAL REVIEWS IN MICROBIOLOGY
Volume 37, Issue 1, Pages 15-24Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/1040841X.2010.506177
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Funding
- U.S. Environmental Protection Agency (EPA) through its Office of Research and Development and Housing and Urban Development (HUD)
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Traditional environmental mould analysis is based on microscopic observations and counting of mould structures collected from the air on a sticky surface or culturing of moulds on growth media for identification and quantification. These approaches have significant limitations. A DNA-based method of mould analysis called mould specific quantitative PCR (MSQPCR) was created for more than 100 moulds. Based on a national sampling and analysis by MSQPCR of dust in US homes, a scale for comparing the mould burden in homes was created called the Environmental Relative Mouldiness Index (ERMI).
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