Journal
CRITICAL REVIEWS IN BIOTECHNOLOGY
Volume 33, Issue 1, Pages 1-22Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/07388551.2012.659173
Keywords
Adsorbent; activated charcoal; dimethylpolysiloxane; lichroprep RP-8; lipophilic; liquid-liquid system; liquid paraffin; liquid-solid system; miglyol; plant cell culture; silicon oil
Categories
Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2009/53450-8]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Spanish MEC [BIO2008-01210]
- Calatan Government [2009SGR1217]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [09/53450-8] Funding Source: FAPESP
Ask authors/readers for more resources
The two-phase culture system is an important in vitro strategy to increase the production of secondary metabolites (SMs) by providing an enhanced release of these compounds from plant cells. Whereas the first phase supports cell growth, the second phase provides an additional site or acts as a metabolic sink for the accumulation of SMs and also reduces feedback inhibition. This review is focused on several aspects of the two-phase culture system and aims to show the diverse possibilities of employing this technique for the in vitro production of SMs from plant cells. Depending on the material used in the secondary phase, two-phase culture systems can be broadly categorised as liquid-liquid or liquid-solid. The choice of material for the second phase depends on the type of compound to be recovered and the compatibility with the other phase. Different factors affecting the efficiency of two-phase culture systems include the choice of material for the secondary phase, its concentration, volume, and time of addition. Factors such as cell elicitation, immobilization, and permeabilization, have been suggested as important strategies to make the two-phase culture system practically reliable on a commercial scale. Since there are many possibilities for designing a two-phase system, more detailed studies are needed to broaden the range of secondary phases compatible with the various plant species producing SMs with potential applications, mainly in the food and pharmacology industries.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available