Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume 15, Issue 9, Pages 6444-6451Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2015.10709
Keywords
Nanoceria; Bone Marrow Stromal Cells; Proliferation; Osteogenic Differentiation; Adipogenic Differentiation
Categories
Funding
- Chinese Natural Science Foundation [21271059]
- Research Fund for the Doctoral Program of Higher Education of China [20111301110004]
- Hebei Province Hundred Talents Program [BR2-202]
- Training Program for Innovative Research Team and Leading Talent in Hebei Province University [LJRC024]
Ask authors/readers for more resources
The effects of cerium oxide nanoparticles (nanoceria) on the proliferation, osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells (BMSCs) were studied by employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MU), alkaline phosphatase (ALP) activity, collagen production, alizarin red-S (ARS) and oil red o stain assays. The results indicated that nanoceria increased the viability of BMSCs at all tested concentrations with evident dose dependence for 24 and 72 h. On day 14, nanoceria inhibited the osteogenic differentiation of BMSCs at all tested concentrations. On day 19 and 24, nanoceria inhibited the formation of mineralized matrix nodules of BMSCs at all tested concentrations. On day 17, nanoceria inhibited the adipogenic differentiation of BMSCs at all tested concentrations. This suggests that the effects of nanoceria on the proliferation, osteogenic differentiation and adipogenic differentiation of BMSCs are very complicated. Both the concentration and culture time have significant influence on the proliferation, osteogenic differentiation and adipogenic differentiation of BMSCs. These results will be helpful for rational applications of nanoceria in the future.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available