Journal
COORDINATION CHEMISTRY REVIEWS
Volume 257, Issue 2, Pages 315-331Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.ccr.2012.05.020
Keywords
Molybdenum; Tungsten; Nitrate reductase; Formate dehydrogenase; Catalytic mechanism; Metal selectivity; MoCo/WCo biosynthesis
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Funding
- Fundacao para a Ciencia e a Tecnologia (FCT) [PEst-C/EQB/LA0006/2011]
- FCT
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Mo- and W-enzymes are widely distributed in biology as they can be found in all domains of life. They perform key roles in several metabolic pathways catalyzing important reactions of the biogeochemical cycles of the more abundant elements of the earth. These reactions are usually redox processes involving the transfer of an atom from the substrate to the metal ion or vice versa. The Mo or W reactivity and specificity toward a substrate is determined by the polypeptide chain of the enzyme, which tunes the chemical properties of the metal ion. Two enzymes sharing almost identical active sites but catalyzing very different reactions are periplasmic nitrate reductase and formate dehydrogenase from bacteria. They represent a good example of how key changes in the amino acid sequence tune the properties of an enzyme. In order to analyze the chemistry of Mo and W in these enzymes, structural, kinetic and spectroscopic data are reviewed, along with the role of these enzymes in cell metabolism. In addition, the features that govern selectivity of metal uptake into the cell and Mo/W-cofactor biosynthesis are revised. (C) 2012 Published by Elsevier B.V.
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