Journal
CONTRAST MEDIA & MOLECULAR IMAGING
Volume 8, Issue 1, Pages 57-62Publisher
WILEY-HINDAWI
DOI: 10.1002/cmmi.1494
Keywords
dynamic nuclear polarization; relaxation; hyperpolarization; pyruvate
Funding
- Ontario Institute for Cancer Research
- Imaging Translation Program
- Natural Sciences and Engineering Research Council of Canada
Ask authors/readers for more resources
In vivo metabolism of hyperpolarized pyruvate has been demonstrated to be an important probe of cellular glycolysis in diseases such as cancer. The usefulness of hyperpolarized C-13 imaging is dependent on the relaxation rates of the C-13-enriched substrates, which in turn depend on chemical conformation and properties of the dissolution media such as buffer composition, solution pH, temperature and magnetic field. We have measured the magnetic field dependence of the spin-lattice relaxation time of hyperpolarized [1-C-13]pyruvate using field-cycled relaxometry. [1-C-13]pyruvate was hyperpolarized using dynamic nuclear polarization and then rapidly thawed and dissolved in a buffered solution to a concentration of 80 mmol l(-1) and a pH of similar to 7.8. The hyperpolarized liquid was transferred within 8 s to a fast field-cycling relaxometer with a probe tuned for detection of C-13 at a field strength of similar to 0.75 T. The magnetic field of the relaxometer was rapidly varied between relaxation and acquisition fields where the sample magnetization was periodically measured using a small flip angle. Data were recorded for relaxation fields varying between 0.237 mT and 0.705 T to map the T-1 dispersion of the C-1 of pyruvate. Using similar methods, we also determined the relaxivity of the triarylmethyl radical (OX063; used for dynamic nuclear polarization) on the C-1 of pyruvate at field strengths of 0.001, 0.01, 0.1 and 0.5 T using 0.075, 1.0 and 2.0 mmol l(-1) concentrations of OX063 in the hyperpolarized pyruvate solution. Copyright (C) 2012 John Wiley & Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available