Journal
CONTRAST MEDIA & MOLECULAR IMAGING
Volume 4, Issue 5, Pages 230-236Publisher
WILEY-HINDAWI
DOI: 10.1002/cmmi.289
Keywords
cell tracking; SPIO labeling; MRI; iron oxide labeling; ferumoxides; protamine sulfate; MSC; chondrocyte
Funding
- European Network for Cell Imaging and Tracking Expertise [Helath-F5-2008-0201842]
- Netherlands Ministry of Economic Affairs
- Netherlands Ministry of Education, Culture and Science
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The use of superparamagnetic iron oxide (SPIO) for labelling cells holds great promise for clinically applicable cell tracking using magnetic resonance imaging. For clinical application, an effectively and specifically labeled cell preparation is highly desired (i.e. a large amount of intracellular iron and a negligible amount of extracellular iron). In and clinically translatable approaches. These approaches are cell labeling using ferumoxides-protamine complexes of ferucarabotran particles. Cell labeling was performed on primary human bone marrow stromal cells (hBMSCs) and chondrocytes. For both cell types ferumoxides-protamine resulted in a higher percentage of labeled cells, a higher total iron load, a larger amount of intracellular iron and a lower amount of extracellular iron aggregates, compared with ferucarbotran. Consequently, hBMSC and chondrocyte labeling with ferumoxides-protamine is more effective and results in more specific cell labeling than ferucarbotran. Copyright (C) 2009 John Wiley & Sons, Ltd.
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