Journal
ANIMAL REPRODUCTION SCIENCE
Volume 159, Issue -, Pages 172-183Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.anireprosci.2015.06.015
Keywords
Bovine; Blastocyst; PRDX II; Apoptosis; Mitochondria; Gene expression
Funding
- Next-Generation BioGreen 21 program [PJ01107703]
- RDA [PJ00932101]
- Ministry of Education, Republic of Korea.
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Endogenous peroxiredoxin II (PRDX II) protein plays a vital role in early embryonic development. This study assessed the beneficial effects of exogenous PRDX II on bovine embryo development at the cellular and molecular levels. To this end, in vitro maturation (IVM) medium was supplemented with various concentrations of PRDX 11 (0, 6.25, 12.5, 25, 50, and 100 mu g/mL). Of these, 12.5 mu g/mL PRDX II was the most effective and significantly promoted embryonic development. Therefore, this concentration of PRDX II was used in subsequent experiments. The percentage of embryos that developed into Day 8 blastocysts and the total number of cells per blastocyst (38.2% and 150.6 +/- 5.1) was higher in the PRDX II-treated group than in the control (26.4% and 128.9 +/- 3.9, respectively). Moreover, the percent of TUNEL positive cells was higher (P<0.05) in the control than in the PRDX II-treated. Furthermore, PRDX II added to the IVM media increased mitochondria content in blastocysts and decreased the intracellular ROS levels in oocytes and blastocysts compared with the control (P<0.05). The expression of genes associated with blastocyst quality (CDX2 and IFN tau), antioxidant activity (SOD2), and mitochondrial activity (TFAM) was higher, whereas the expression of a gene involved in the apoptotic pathway (c-FOS) was lower, in the PRDX II-treated than in the control group. In conclusion, supplementation of IVM medium with PRDX II promotes development to the blastocyst stage and improves blastocyst quality through reducing ROS, enhancing embryonic mitochondrial activity, and modulating development-related target genes expression. (C) 2015 Elsevier B.V. All rights reserved.
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