Journal
JOURNAL OF MOLECULAR NEUROSCIENCE
Volume 57, Issue 3, Pages 426-434Publisher
HUMANA PRESS INC
DOI: 10.1007/s12031-015-0589-3
Keywords
Neovascularization; Endothelial progenitor cell; IGF binding protein 2; Integrin; RGD motif
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Funding
- science and technology project of the Education Department of Heilongjiang Province [12521336]
- Health Department of Heilongjiang Province [2011-089]
- Foundation of the Second Clinical Hospital of Harbin Medical University [QN2011-18]
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The contribution of endothelial progenitor cells (EPCs) to new vessel formation has been studied in different physiological and pathological conditions for decades. As previously suggested, insulin-like growth factor binding protein-2 (IGFBP-2) may interact with integrins and promote cell migration. However, the role of IGFBP-2 in regulation of EPC functions remains largely unknown. In this present study, we found that overexpression of IGFBP-2 in human umbilical vein endothelial cells (HUVECs) promoted EPC-endothelial adhesion. Conversely, siRNA-mediated depletion of IGFBP-2 inhibited oxygen-glucose deprivation (OGD)-induced EPC-endothelial adhesion. Further, we demonstrated that the arginine-glycine-aspartic acid (RGD) motif in its C-domain is required for interaction with integrin alpha 5 beta 1. In addition, treatment with IGFBP-2 significantly enhanced incorporation of EPCs into tubule networks formed by HUVECs. Thus, our findings suggest that exogenous administration of IGFBP-2 may facilitate neovascularization and improve treatment of ischemic conditions.
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