Journal
CONNECTIVE TISSUE RESEARCH
Volume 55, Issue -, Pages 33-37Publisher
TAYLOR & FRANCIS INC
DOI: 10.3109/03008207.2014.923883
Keywords
Basement membrane; dentin enamel junction; mature human enamel; MMP-20; type IV collagen; type VII collagen
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Funding
- NIH/NIDCR [R01-DE021462, F32-DE022984]
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Although mature enamel is predominantly composed of mineral, a previously uncharacterized organic matrix layer remains in the post-eruptive tissue that begins at the dentin enamel junction and extends 200-300 mu m towards the outer tooth surface. Identification of the composition of this layer has been hampered by its insolubility; however, we have developed a single step method to isolate the organic enamel matrix relatively intact. After dissociative dissolution of the matrix with SDS and urea, initial characterization by Western blotting and gel zymography indicates the presence of type IV and type VII basement membrane collagens and active matrix metalloproteinase-20. When combined with data from transgenic knockout mice and from human mutations, these data suggest that the enamel organic matrix (EOM) and dentin enamel junction may have a structural and functional relationship with basement membranes, e. g. skin. To clarify this relationship, we hypothesize a foundation model which proposes that components of the EOM form a support structure that stabilizes the crystalline enamel layer, and bonds it to the underlying dentin along the dentin enamel junction. Since we have also co-localized an active matrix metalloproteinase to this layer, our hypothesis suggests that, under pathologic conditions, MMP-mediated degradation of the EOM could destabilize the enamel-dentin interface.
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