Journal
JOURNAL OF MOLECULAR NEUROSCIENCE
Volume 57, Issue 1, Pages 38-47Publisher
HUMANA PRESS INC
DOI: 10.1007/s12031-015-0575-9
Keywords
Nur77; Schwann cells; Differentiation; PC12 cells; Outgrowth; Sciatic nerve crush
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Funding
- National Natural Science Foundation of China [81171140, 81471258]
- Colleges and Universities in Natural Science Research Project of Jiangsu Province [13KJB31009]
- Priority Academic Program Development of jiangsu Higher Education Institution (PAPD)
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Nur77, together with Nurr1 and NOR-1, constitutes the NR4A subgroup of orphan nuclear receptors and plays critical roles in cell proliferation, differentiation, migration, and apoptosis. Among them, Nur77 is universally well known to contribute to neurite outgrowth. However, information regarding its regulation and possible function in the peripheral nervous system is still limited. In this study, we performed a sciatic nerve injury model in adult rats and detected an increased expression of Nur77 in the sciatic nerve, which was similar to the expression of Oct-6. Immunofluorescence indicated that Nur77 was located in both axons and Schwann cells. In vitro, we observed enhanced expression of Nur77 during the process of both basic fibroblast growth factor (bFGF)-induced Schwann cells differentiation and nerve growth factor (NGF)-induced PC12 cell neurite outgrowth. In vitro and in vivo experiments indicated that inhibiting the function of Nur77 by specific short hairpin RNA could depress Schwann cells myelinization and axons regeneration. Collectively, all these results suggested that upregulation of Nur77 might be involved in Schwann cells differentiation and neurite elongation following sciatic nerve crush.
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