Journal
CONCEPTS IN MAGNETIC RESONANCE PART A
Volume 32A, Issue 3, Pages 183-200Publisher
WILEY-HINDAWI
DOI: 10.1002/cmr.a.20109
Keywords
13C direct-detected NMR; hymenistatin; protonless NMR; spin-systems assignment; sequential assignment; spin-state selection; ubiquitin
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Carbon-13 direct-detection NMR is becoming increasingly used to complement H-1-detected experiments in biomolecular applications. Here we describe the novel aspects that were addressed to convert C-13 direct-detection into a useful routinely applicable tool, a selection of the most useful C-13 direct-detection protonless NMR experiments and how they can be exploited for the study of proteins. (C) 2008 Wiley Periodicals, Inc.
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