Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 116, Issue -, Pages 166-172Publisher
ELSEVIER
DOI: 10.1016/j.molcatb.2015.03.019
Keywords
Polysaccharides; Polysaccharide hydrolase; Polysaccharide lyase; Enzyme Ring Test (R)
Funding
- French government research program Investissements d'avenir through the IMobS3 Laboratory of Excellence [ANR-10-LABX-16-01]
- European Union through the program Regional competitiveness and employment (ERDF-Auvergne region) [mandat Etat 210013832 Ex.2013 / Region 210011493 Ex.2012]
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Most of usual methods used to detect and quantify polysaccharide cleavage enzymes (PCE) are not adapted to high throughput. In this work, enzymatic activities of three enzymes, namely an endocellulase (EC 3.2.1.4), an exocellulase (EC 3.2.1.91) and an alginate lyase (EC 4.2.2.3) were characterized by classical Michaelian model (K-m, V-max). Enzymatic activities were then determined by the use of the Enzyme Ring Test (R) (ERT (R)) method which allowed following the depolymerization of polysaccharides by PCE. Kinetic parameters were determined and compared to the ones obtained by classical methods. Besides, the ERT (R) technology clearly showed the possibility to discriminate endolytic and exolytic enzymatic mechanisms. (C) 2015 Elsevier B.V. All rights reserved.
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