4.5 Article

Two mutations in the 5′-flanking region of the KITLG gene are associated with litter size of dairy goats

Journal

ANIMAL GENETICS
Volume 46, Issue 3, Pages 308-311

Publisher

WILEY-BLACKWELL
DOI: 10.1111/age.12277

Keywords

candidate gene; combined genotype; primer-introduced restriction analysis-polymerase chain reaction; single nucleotide polymorphism

Funding

  1. National Support Program of China [2011BAD28B05-3]
  2. National Sparking Plan Project [2013GA850003]
  3. Science and Technology Innovation Project of Shaanxi Province [2011KTCL02-09]
  4. Northwest AF University [2013BSJJ003]
  5. China Postdoctoral Science Foundation [133783]

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In this study, Xinong Saanen (SN) and Guanzhong (GZ) dairy goat breeds were used to detect single nucleotide polymorphisms (SNPs) in the 5-flanking region of the KITLG gene by DNA sequencing and primer-introduced restriction analysis-polymerase chain reaction. Two novel SNPs (g.13090G>T and g.13664C>A) were identified (GenBank Accession no. KM658964). Furthermore, g.13090G>T and g.13664C>A loci were closely linked in SN and GZ breeds (r(2)>0.33). Association analysis results showed that g.13090G>T and g.13664C>A SNPs significantly affected litter size (P<0.05). The litter size of individuals with the combined genotype GG/CC from both dairy goat breeds was greater than that of individuals with TT/AA in average parity (P<0.05). Known biochemical and physiological functions, along with our results, indicated that GG/CC could be used in marker-assisted selection to choose individuals with greater litter size from both breeds. These results extend the spectrum of genetic variation in the caprine KITLG gene and may contribute to genetic resources and breeding of goats.

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