Journal
ANIMAL FEED SCIENCE AND TECHNOLOGY
Volume 208, Issue -, Pages 119-131Publisher
ELSEVIER
DOI: 10.1016/j.anifeedsci.2015.07.001
Keywords
Bacillus amyloliquefaciens; Immunological stress; Intestinal damage; Antioxidant status; Broiler
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Funding
- National Science-Technology Support Plan Projects of China [2013BAD10B02-03]
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The present study investigated the effects of Bacillus amyloliquefaciens (BA) supplementation on the growth performance, intestinal integrity, antioxidant and immune status of broilers challenged with lipopolysaccharide (LPS). 192 one-day-old. male Arbor Acre broilers were randomly distributed into four treatments: (1) non-challenged control; (2) LPS-challenged control; (3) LPS-challenged group + 0.5 g/kg of diet; and (4) LPS-challenged group + 1.0 g/kg of diet. Broilers were intraperitoneally injected with either 500 mu g/kg body weight of LPS or sterile saline at 16, 18 and 20 d of age. The inclusion of 1.0 g/kg BA alleviated the compromised average daily gain caused by LPS challenge, the body weight gain in 1.0 g/kg BA supplemented group was still significantly lower than that of control (P< 0.05). Similarly, BA supplementation attenuated intestinal morphology impairment, and reduced intestinal malonaldehyde concentration and myeloperoxidase activity after LPS injection (P< 0.05). The 1.0 g/kg BA inclusion prevented the elevation of circulating diamine oxidase activity and reduction of intestinal glutathione concentration induced by LPS challenge (P< 0.05). The BA decreased interleukin (IL)-1 beta, whereas increased IL-10 at both protein and transcriptional levels in jejunum, and attenuated LPS-induced elevated mRNA expression of jejunal toll-like receptor 4 (P< 0.05). Moreover, inclusion of 1.0 g/kg BA alleviated LPS-induced negative effect on the mRNA abundance of tight junctions (P< 0.05). It can therefore be postulated that BA supplementation alleviates LPS-induced intestinal mucosal damage by improving intestinal integrity and antioxidant and immune status. (C) 2015 Elsevier B.V. All rights reserved.
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