Journal
COMPUTATIONAL BIOLOGY AND CHEMISTRY
Volume 33, Issue 1, Pages 84-93Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.compbiolchem.2008.07.006
Keywords
MicroRNA; Solanum tuberosum; Computational analyses; Transcription factor
Funding
- National Natural Science Foundation of China [30660042]
- Natural Science Foundation of Jiangxi province [0630136, 0630137]
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MicroRNAs (miRNAs) are a new family of small RNA molecules known in animals and plants, whose conservation among species suggests that they bear conserved biological functions. So far, little is known about miRNA in Solanum tuberosum species. Using previously known miRNAs from Arabidopsis, rice and other plant species against expressed sequence tags (ESTs), genomic survey sequence (GSS) and nucleotide databases, we identified 48 potential miRNAs in S. tuberosum. These potato miRNAs may regulate 186 potential targets, which are involved in floral, leaf, root, and stem development, signal transduction, metabolism pathways, and stress responses. To validate the prediction of miRNAs in potato,we performed a RT-PCR analysis and found that potato miRNAs have diverse expression patterns during development. (c) 2008 Elsevier Ltd. All rights reserved.
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