Journal
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 25, Issue 2, Pages 268-273Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1408.08048
Keywords
Varicella-zoster virus; fluorescent-antibody-to-membrane-antigen test; chickenpox; shingles; glycoprotein E
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Funding
- National Research Foundation of Korea - Ministry of Education (LINC) [2014-D-7266-010103]
- Leading Space Core Technology Development Program through the NRF - Ministry of Science, ICT, & Future Planning (MSIP) [2013-042433]
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The fluorescent-antibody-to-membrane-antigen (FAMA) test is regarded as the gold standard to detect protective antibodies to varicella-zoster virus (VZV) because of its high sensitivity and specificity. Because the classic FAMA test uses an infectious virus for detection of antibodies to VZV, it is labor-intensive, and also requires special equipment for handling the virus. For this reason, we attempted to develop a simple and safe FAMA assay. Because VZV glycoprotein E (gE) is one of the major VZV glycoproteins, we used the gE protein for the FAMA test (gE FAMA). Here, we demonstrate that overexpression of gE in HEK293T cells can be used to measure antibodies in human serum, and that gE FAMA titers are closely correlated with gpEIA ELISA data. These results indicate that our gE FAMA test has the potential to measure antibodies to VZV.
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