Journal
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 25, Issue 10, Pages 1648-1652Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1504.04086
Keywords
Monascus pigment; azaphilone polyketide; non-reducing fungal polyketide synthase; MppD; Ser/Asp/His catalytic triad
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2013R1A1A2059458]
- National Research Foundation of Korea [2013R1A1A2059458] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Azaphilone polyketides are synthesized by iterative non-reducing fungal polyketide synthases (NR-fPKSs) with a C-terminus reductive domain (-R). Several azaphilone biosynthetic gene clusters contain a putative serine hydrolase gene; the Monascus azaphilone pigment (MAzP) gene cluster harbors mppD. The MAzP productivity was significantly reduced by a knockout of mppD, and the MAzP NR-fPKS-R gene (MpPKS5) generated its product in yeast only when co-expressed with mppD. Site-directed mutations of mppD for conserved Ser/Asp/His residues abolished the product formation from the MpPKS5/mppD co-expression. MppD and its homologs are thus proposed as a new protein factor involved in the product formation of NR-fPKS-R.
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