Journal
JOURNAL OF MICROBIOLOGY
Volume 53, Issue 4, Pages 236-242Publisher
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-015-4639-x
Keywords
protein kinase; vegetative growth; conidiation; stress response; induced cellulase production
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Funding
- National Basic Research Program of China [2011CB707402]
- National Natural Science Foundation [31270116, 31470162]
- New Century Excellent Talents in University [NCET-10-0546]
- Shandong Provincial Funds for Distinguished Young Scientists [JQ201108]
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Protein kinases are key players in controlling many basic cellular processes in almost all the organisms via mediating signal transduction processes. In the present study, we characterized the cellulolytic Trichoderma reesei orthologs of Saccharomyces cerevisiae Sch9 and Yak1 by sequence alignment and functional analysis. The T. reesei Trsch9 Delta and Tryak1 Delta mutant strains displayed a decreased growth rate on different carbon sources and produced less conidia. The absence of these two kinases also resulted in different but abnormal polarized apical growth as well as sensitivity to various stresses. In addition, disruption of the genes Trsch9 or Tryak1 resulted in perturbation of cell wall integrity. Interestingly, while the induced production of cellulases was slightly compromised in the Trsch9 Delta strain, the extracellular production of cellulases was significantly improved in the absence of Yak1. The results indicate that TrSch9 and TrYak1 play an important role in filamentous growth, stress response and induced production of cellulases in T. reesei.
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