Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 115, Issue -, Pages 67-70Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2015.05.024
Keywords
Fungal pretreatment; Solid State Fermentation (SSF); Plant biomass
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Funding
- French National Research Agency Program Bio-ME [ANR-12-BIME-0009]
- Agence Nationale de la Recherche (ANR) [ANR-12-BIME-0009] Funding Source: Agence Nationale de la Recherche (ANR)
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Filamentous fungi have shown great potential in the pretreatment of lignocellulosic biomass and their use in bio-processes based on Solid State Fermentation (SSF) opens promising perspectives for plant biomass valorization. Obviously, quantification of the fungal biomass throughout the fermentation is a crucial parameter for SSF evaluation and control, both at the laboratory and industrial scale. Here we provide a qPCR-based method as a reliable alternative to conventional methods to estimate mycelial growth during plant biomass treatment. For the three strains analyzed, the lowest detection limit ranged from 58 to 272 mu g mycelium dry weight per gram biomass (dry weight). We show that the qPCR-based method allows fungal growth monitoring during fermentation and provides relevant information for selection of the most appropriate fungal strains in specific SSF/reactor conditions. (C) 2015 Elsevier B.V. All rights reserved.
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