4.3 Article

Fast and reliable bacterial identification direct from positive blood culture using a new TFA sample preparation protocol and the Vitek® MS system

Journal

JOURNAL OF MICROBIOLOGICAL METHODS
Volume 109, Issue -, Pages 157-159

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ELSEVIER
DOI: 10.1016/j.mimet.2014.12.009

Keywords

Blood culture; Mass spectrometry; MALDI-TOF; Vitek MS; Trifluoroacetic acid; Bacterial identification

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A simple and reliable protocol using 0.1% trifluoroacetic acid (TFA) was developed to identify bacteria directly from blood cultures on the Vitek (R) MS system. Results presented a correlation of 99% for Gram-negative bacteria at species level, and 863% and 82.3% of Gram-positive bacteria at the genus and species levels, respectively. (C) 2014 Elsevier B.V. All rights reserved.

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