Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 118, Issue -, Pages 173-175Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2015.09.019
Keywords
Antibacterial assay; Combination testing; DMSO tolerance; HTS
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A 384-well-based antibacterial assay amenable for high-throughput screening and combination testing is described. The assay uses 100-500 nL of test compounds and tolerates up to 2.5% dimethyl sulfoxide concentrations. It can be used for screening compound libraries and testing combinatory/synergistic/antagonistic effects of antibiotics, small molecules, and natural product extracts. (C) 2015 Elsevier B.V. All rights reserved.
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