Journal
COMPTES RENDUS BIOLOGIES
Volume 334, Issue 11, Pages 812-823Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.crvi.2011.06.005
Keywords
Lignocellulose; Steam pretreatment; Enzyme adsorption; Poly(ethylene glycol); Lignin; Cellulase; beta-glucosidase
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Funding
- European Cooperation [FP0602]
- Hungarian National Research Fund (OTKA) [K 72710, K 81959]
- New Hungary Development Plan [TAMOP-4.2.1/B-09/1/KMR-2010-0002]
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The efficiency of enzymatic hydrolysis of lignocellulses can be increased by addition of surfactants and polymers, such as poly(ethylene glycol) (PEG). The effect of PEG addition on the cellulase adsorption was tested on various steam pretreated lignocellulose substrates (spruce, willow, hemp, corn stover, wheat straw, sweet sorghum bagasse). A positive effect of PEG addition was observed, as protein adsorption has decreased and free enzyme activities (FP. beta-glucosidase) have increased due to the additive. However, the degree of enhancement differed among the substrates, being highest on steam pretreated spruce. Results of lignin analysis (pyrolysis-GC/MS, P-31 NMR) suggest that the effect of PEG addition is in connection with the amount of unsubstituted phenolic hydroxyl groups of lignin in the substrate. Adsorption experiments using two commercial enzyme preparations. Celluclast 1.5L (Trichoderma reesei cellulase) and Novozym 188 (Aspergillus niger beta-glucosidase) suggested that enzyme origins affected on the adsorptivity of beta-glucosidases. (C) 2011 Academie des sciences. Published by Elsevier Masson SAS. All rights reserved.
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