Comparative effect of potassium on K and Na uptake and transport in two accessions of Arabidopsis thaliana during salinity stress

Potassium-sodium interaction was compared in two natural accessions of Arabidopsis thaliana, Columbia-0 and NOK2. Seedlings were grown in the presence of 0 or 50 mM NaCl and 0.1; 0.625 or 2.5 mM K+. At the lowest K+ concentration, salt treatment inhibited both K+ uptake and growth. Increasing the K+ availability did not modified salt response in Columbia-0, but restored nearly normal net K+ uptake in NaCl condition and alleviated NaCl growth reduction in NOK2. The effect of K+ and NaCl on transcript level of several K+ and Na+ transporters in both shoots and roots was assessed using semi-quantitative RT-PCR. The mRNA abundance of the NHX1 and SOS1 Na+/H+ antiporters was significantly increased by 50 mM NaCl in the two accessions. NHX1, which is responsible for Na+ sequestration into vacuoles, was more up-regulated in NOK2 leaves than in Columbia-0's in NaCl stress condition. AKT1, which is the major channel involved in K+ absorption, was down-regulated in salt stress condition, but was not responding to K+ treatments. Only in NOK2, SKOR and AKT2, which respectively control xylem and phloem K+ transport, were markedly up-regulated by 2.5 mM K+ in both roots and shoots, independently of NaCl. Phenotypic and gene expression analyses suggest that the relative salt tolerance of NOK2 is mainly due to a high ability to sequester Na+ in the vacuole and to take up and transport K+. Up-regulation of SKOR and AKT2 by K+, and of NHX1 by NaCl could participate in determining this phenotype. To cite this article: R. Kaddour et al., C R. Biologies 332 (2009). (C) 2009 Published by Elsevier Masson SAS on behalf of Academie des sciences.