Journal
COMPOSITES SCIENCE AND TECHNOLOGY
Volume 69, Issue 3-4, Pages 539-544Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.compscitech.2008.11.017
Keywords
Hybrid composite; Functional composite; Recycling; Polymer-matrix composite; Polymer
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Funding
- Natural Science Foundation of Tianjin [06YFJMJC10600, 09YFJMJC]
- Cross-Century Talent Raising Program of Ministry of Education of China
- program for Changjiang Scholars and Innovative Research Team in University (PCSIRT)
- National Science Foundation of China [20576096]
- Program of Introducing Talents of Discipline to Universities [1306006]
- State Key Laboratory of Fine Chemicals, Dalian University of Technology
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Mesoporous CaCO3 microparticle-doped alginate composite gels were prepared and studied as efficient carriers for enzyme immobilization. beta-Glucuronidase (GUS) was immobilized by first pre-adsorption on CaCO3 microparticles and then encapsulation in alginate beads. In this hybrid composite, alginate matrix effectively prevented the desorption of enzyme and inhibited the recrystallization of CaCO3, while the impregnated CaCO3 particles remarkably reduced the swelling of alginate hydrogel from 130% to 20%. As a result, GUS loading efficiency, recycling and storage stability were all significantly improved. Immobilized GUS retained 80% of its initial activity after seven recycles and 67% after storage for 27 days. (C) 2008 Elsevier Ltd. All rights reserved.
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