Journal
JOURNAL OF MEMBRANE SCIENCE
Volume 473, Issue -, Pages 102-108Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.memsci.2014.08.059
Keywords
Plasmid; DNA; Ultrafiltration; Fouling; Backpuising
Categories
Funding
- National Science Foundation [CBET 0755816]
Ask authors/readers for more resources
There is considerable interest in using ultrafiltration for the purification of plasmid DNA, but membrane fouling remains an issue. The objective of this work was to examine the use of periodic backpulsing reduce fouling during plasmid ultrafiltration by releasing previously trapped plasmids from the membrane pores. Experiments were performed using hollow fiber ultrafiltration membranes over a range of backpulse frequency, amplitude, and duration. Plasmid removal under typical conditions was dominated by the shear / drag associated with the tangential flow, with good recovery of plasmid transmission obtained by periodically closing the permeate exit line (without any backpressure). Data were analyzed using a simple fouling model that accounts for the partial blockage of pores by trapped plasmids that are then released during backpulsing. The results clearly demonstrate that this type of pulsed operation can provide much greater plasmid recovery in the permeate solution with a nearly constant filtrate flux during a dial-titration process. (C) 2014 Elsevier B.V. All rights reserved
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available