Journal
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
Volume 33, Issue 2, Pages 161-174Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cimid.2008.08.003
Keywords
Porcine intestinal epithelial cell lines; LPS; TLR; Cytokines; Chemokines
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Funding
- EU
- SABRE
- Spanish Ministry of Education and Science
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The innate immune system has the basic function of identifying and eradicating microbial invaders and alerting the adaptative immune system to their presence. In this study, the porcine intestinal innate immune response was evaluated by analysing the expression of TLRs, cytokines and chemokines in two porcine epithelial cell lines from different regions: IPEC-J2 (jejunum) and IPI-2I (ileum). Both cells lines were stimulated with 1 mu g of LIPS from Salmonella typhimurium. RNA was collected at 30 min, 1, 2, 3 and 4 h after treatment. Expression of TLR-1, -2, -3, -4, -6, -8, -9, -10, TNF-alpha, IL-1 beta, -8 and MCP-1 was quantified relative to the quantity of Cyclophilin-A mRNA using real-time quantitative PCR (RTQ-PCR). The results obtained show up differences ill the gene expression between both cell lines IPEC-J2 and IPI-2I as response to LPS from S. typhimurium during the activation time, which may suggest an in vivo variability in the innate immune response against pathogens in different regions of the host's gut. (C) 2008 Elsevier Ltd. All rights reserved.
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