Journal
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
Volume 33, Issue 6, Pages E67-E73Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cimid.2010.01.001
Keywords
Hantavirus; TULV; PUUV; Japanese grass vole; IFA; ELISA; RT-PCR; Microtus; Myodes; HFRS
Categories
Funding
- Global COE Program, Establishment of International Collaboration Center for Zoonosis Control''
- Ministry of Education, Culture, Sports, Science and Technology, Japan
- Grant for Research on Emerging and Re-emerging Infectious Diseases from Ministry of Health, Welfare, and Labor of Japan
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Tula virus (TULV) and Puumala virus (PUUV) are hantaviruses carried by the bank vole (Myodes glareolus) and European common vole (Microtus arvalis), respectively. PUUV is a causative agent of hemorrhagic fever with renal syndrome (HFRS), while TULV is thought to be apathogenic to humans. The N-terminal regions of the N proteins from TULV and PUUV were expressed and applied as enzyme-linked immunosorbent assay (ELISA) antigens. Colonized Japanese grass voles (Microtus montebelli) and BALB/c mice were used for experimental inoculation of the vole-borne hantaviruses TULV and PUUV. Voles and mice showed significant antibody production toward both viruses, but these antisera showed little cross-reactivity between TULV and PUUV in the immunofluorescence antibody assay and ELISA. In contrast, sera from patients with HFRS caused by PUUV exhibited high cross-reactivity against the TULV antigen, and sera from a natural rodent reservoir showed moderate cross-reactivity against the heterologous antigen, indicating that the antigenic cross-reactivity between TULV and PUUV differs in sera from rodents and humans. (C) 2010 Elsevier Ltd. All rights reserved.
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