Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
Volume 154, Issue 1, Pages 36-41Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpc.2011.02.007
Keywords
Litopenaeus vannamei; Temperature; Oxidative stress; DNA damage; Osmolality
Funding
- National Natural Science Foundation of China [30970455]
- Natural Science Foundation of Guangdong Province, P.R. China [8151063101000035]
- Guangdong Provincial Oceanic Fisheries Science and Technology Project [A200899D01, A200901H06]
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To evaluate the genotoxic, physiological and immunological effects of short-term acute low temperature stress on the Pacific white shrimp, Litopenaeus vannamei, we rapidly transferred shrimp from tanks at 23 +/- 2 degrees C to aquaria at the same temperature (controls) or 12 +/- 2 degrees C for 12 h. Changes in the shrimp hemocyte respiratory burst activity and DNA damage were examined during and after exposure to the temperature stress using flow cytometry and the comet assay, respectively. We also monitored changes in the total hemocyte count malondialdehyde levels, total protein concentration and osmolality in shrimp plasma The results show that hemocyte respiratory burst activity, malondialdehydes levels and hemocyte DNA damage in the plasma all increased significantly after exposure to 12 +/- 2 degrees C for 3 h. In contrast, total hemocyte count total protein concentration and osmolality in the plasma decreased compared to the controls. We conclude that acute low temperature can induce oxidative stress, DNA damage, lipid peroxidation and changes in osmolality in L vannamei. (C) 2011 Elsevier Inc. All rights reserved.
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