Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume 174, Issue -, Pages 23-28Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2014.05.005
Keywords
HO-1; Tibet chicken; Hypoxic adaptation; Expression pattern
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Funding
- National Natural Science Foundation of China [31272401, 91331118]
- Changjiang Scholar and Innovation Research Team in University [IRT1191]
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Heme oxygenase 1 (HO-1), a rate-limiting enzyme of heme catabolism, has a crucial role of cytoprotective functions under hypoxia. The objective of the present study was to investigate potential differences in protective effect of HO-1 gene on chicken (Gallus gallus) embryo lung during late incubation. At embryonic day (D) 016, D18, D19, and D20 of incubation, the expression of HO-1 in the lungs of chicken embryos (Tibet and Shouguang chickens) incubated in normoxic (21% O-2) and hypoxic (13% O-2) conditions was measured. SNPs were screened within 5'-flanking region and coding regions with PCR-sequencing and the genotype of the SNPs was determined with PCR-RFLP in Tibet, Chahua and Shouguang chicken populations. In conclusion, the Tibet chicken had higher HO-1 expression on D19 under hypoxic incubation and had two SNPs with different frequency distributions from other chicken breeds, which might be a way that the Tibet chicken had hereditary adaptation to hypoxia during embryonic development. (C) 2014 Elsevier Inc All rights reserved.
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