Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume 165, Issue 2, Pages 99-105Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2013.03.010
Keywords
Chinese mitten crab; Fatty acyl Delta 6-desaturase-like gene; Cloning; Gene expression; Diet
Categories
Funding
- Innovation Program of Shanghai Municipal Education Commission [11YZ151]
- innovation research group who develop projects in the universities in Shanghai (nutrition, feed, and environment of animal aquaculture)
- Excellent Academic Leaders of Shanghai [12xd1402700]
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Fatty acyl Delta 6-desaturase is the rate-limiting enzyme in the biosynthetic pathway of highly unsaturated fatty acids (HUFAs) in vertebrates. In this report, a fatty acyl Delta 6-desaturase-like cDNA was cloned from the. hepatopancreas of Eriocheir sinensis (Chinese mitten crab) and characterized by performing rapid-amplification of cDNA ends. The 2278-bp long full-length cDNA encodes a polypeptide with 442 amino acids. Gene expression analysis via real-time quantitative polymerase chain reaction revealed that the fatty acyl Delta 6-desaturase-like transcripts are widely distributed in various tissues, with high expression levels in the hepatopancreas and cranial ganglia. This study focuses on the nutritional regulation of genes involved in the HUFA biosynthetic pathway in Chinese mitten crab. A feeding trial was performed whereby crablets were fed for 238 d with four different diets: control diet without oil lipids (added with 3% basic lipid of the fundamental diets); fish oil diet (FO; added with 3% of the fundamental diets); soybean oil diet (SO; added with 3% of the fundamental diets); and FO/SO diet (1:1; added with 3% of the fundamental diets). The hepatopancreas of crabs sampled at 168 d and 238 d to determine the effects on fatty acyl Delta 6-desaturase-like mRNA expression. The results show that the expression of fatty acyl Delta 6-desaturase-like is higher in the hepatopancreas of crabs fed with SO diet than those fed with FO diet. Furthermore, gene expression increased by 2.45-fold in the hepatopancreas of crabs fed with SO after 238 d than those fed after 168 d but remained steady for those fed with FO after 238 d. (C) 2013 Elsevier Inc. All rights reserved.
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