Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume 153, Issue 2, Pages 216-222Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2009.03.003
Keywords
Culex pipiens pallens; Cytochrome P450 6A1; Deltamethrin resistance; Real-time quantitative PCR; Ribosomal protein L22; Transcriptional repression; Mosquito; Insect
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Funding
- National Institutes of Health [1R01AI075746]
- National Natural Science Foundation of China [30671827, 30628022]
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [07KJD180137]
- National Research Foundation for the Doctoral Program of Higher Education of China [20050312004]
- National Infrastructure of Natural Resources for Science and Technology Program of China [2005DKA21104]
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The 60S ribosomal protein L22 (GenBank accession no. EF990190) was cloned from Culex pipiens pallens. An open reading frame (ORF) of 447 bps was found to encode a putative 148 amino acids protein which shares 90% and 80% identity with RPL22 genes from Aedes aegypti and Anopheles gambiae respectively. Real-time quantitative PCR analysis demonstrated that the transcription level of RPL22 in deltamethrin-resistant strain was 2.57 folds higher than in deltamethrin-susceptible strain of Cx pipiens; pallens. Overexpression of RPL22 in C6/36 cells showed that the deltamethrin-resistance was decreased in C6/36-RPL22 cell compared to the control. The mRNA level of cytochrome P450 6A1 (CYP6A1, GenBank accession no. FJ423553) showed that CYP6A1 was down-regulated in the C6/36 transfected with RPL22 (C6/36-RPL22) cells, suggesting that CYP6A1 was repressed by RPL22. Our study provides the first evidence that RPL22 may play some role in the regulation of deltamethrin-resistance in Cx pipiens pallens. (C) 2009 Published by Elsevier Inc.
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