Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume 151, Issue 2, Pages 139-146Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2008.05.012
Keywords
kinetics; metal ion; Ostrinia furnacalis; prophenoloxidase; secondary structure
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Funding
- National Natural Science Foundation of China [30571248]
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Prophenoloxidase (PPO) was isolated from the hemolymph of Ostrinia furnacalis larvae and purified to homogeneity. A 369.85-fold purification and 35.34% recovery of activity were achieved by employing ammonium sulfate precipitation, Blue Sepharose CL-6B chromatography and Phenyl Sepharose CL-4B chromatography. The purified enzyme exhibits a band with a molecular mass of 158 kDa on native PAGE and two spots with a molecular mass of 80 kDa and a pl of 5.70, and a molecular mass of 78 kDa and a pl of 6.50, respectively, on two-dimensional gel electrophoresis. The N-terminal amino acid sequences of two subunits are as follows: PPO1, FGEEPGVQTTELKPLANPPQFRRASQLPRD; PPO2, FGDDAGERIPLQNLSQVPQFRVPSQLPTD. The amino acid composition of purified PPO was similar to that from Galleria mellonella. The enzyme kinetic property of the purified protein showed that the affinity of the enzyme for dopamine was higher than that for L-DOPA and N-acetyldopamine. The phenoloxidase (PO) reaction was strongly inhibited by phenylthiourea, thiourea, dithiothreitol and ethylene diamine tetraacetic acid (EDTA), but poorly inhibited by diethyldithiocarbarnate (DTC) and triethylenetetramine hexaacetic acid (THAA), and was not inhibited by o-phenanthroline and ethylene glycol-bis (beta-aminoethylether) N,N,N',N'-tetraacetic acid (EGTA). Both Mg2+ and Cu2+ stimulated PO activity when compared with controls. The beta-sheet content of PPO treated with Mg2+ and Cu2+ increased significantly (P < 0.05). The purified PPO has magnesium level of 5.674 +/- 2.294 mu g/mg and copper level of 1.257 +/- 0.921 mu g/mg as determined with ICP-MS, suggesting that the purified PPO is a metalloprotein. (C) 2008 Published by Elsevier Inc.
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