Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume 151, Issue 2, Pages 230-235Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2008.07.008
Keywords
Culex pipiens pallens; deltamethrin; MRLC; real-time quantitative PCR; transfection
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Funding
- National Natural Science Foundation of China [30671827, 30628022]
- Science and Technology Development Foundation of Nanjing Medical University [06NMUZ005 to Donghui Zhang]
- National Infrastructure of Natural Resources for Science and Technology Program of China [2005DKA21104]
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Myosin regulatory light chain (MRLC) (GenBank accession no. DQ140391) was cloned from Culex pipiens pallens. An open reading frame (ORF) of 630 bps was found to encode a putative 210 amino acids protein which shows 73% similarity with myosin regulatory light chain of Gryllotalpa orientalis. Real-time quantitative PCR analysis demonstrated that the transcription level of MRLC in deltamethrin-resistant strain (DR-strain) was 4.08-fold higher than in deltamethrin-susceptible strain (DS-strain) of C. pipiens pallens. Over-expression of MRLC in Aedes albopictus C6/36 cells conferred protection against deltamethrin based on tritiated methyl tritiated thymidine (H-3-TdR) incorporation assay. These results indicate that MRLC may be a potential cause of deltamethrin resistance in C. pipiens pallens. (c) 2008 Elsevier Inc. All rights reserved.
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