trans-Resveratrol and ε-viniferin decrease glucose absorption in porcine jejunum and ileum in vitro

trans-Resveratrol and epsilon-viniferin are used as dietary supplements. They are reported to be supportive in preventing arteriosclerosis and diabetes and a previous study could demonstrate an inhibitory potential on sodium-dependent glucose transport (SGLT1) in oocytes und mouse intestinal everted rings (Schulze et al., 2012, Genes Nutr. 6, S61). The in vitro effects of trans-resveratrol and epsilon-viniferin on intestinal glucose uptake in the porcine small intestines (Sus Scrofa) have not yet been evaluated. It was hypothesized that transresveratrol/epsilon-viniferin may have an adverse effect on porcine intestinal sodium-dependent glucose uptake. The effects on electrogenic small intestinal glucose absorption and sodium-dependent H-3-glucose uptake in brush border membrane vesicles (BBMV) were evaluated. Pieces of mucosa were mounted into Ussing chambers and were incubated with either trans-resveratrol (03 mmol/L), epsilon-viniferin (0.3 mmol/L), or ethanol. Sodium-dependent glucose absorption into BBMV was measured. H-3-glucose uptake studies were performed using the same concentrations of the respective substances. SGLT1-mediated glucose absorption was approximately 3-fold higher in ileum compared to jejunum. After preincubation with trans-resveratrol and epsilon-viniferin, glucose-induced increases of short-circuit currents were significantly decreased. BBMV-studies revealed comparable results and glucose uptake was also significantly decreased. As the glucose transport/uptake was decreased after preincubation with either trans-resveratrol or epsilon-viniferin this active transport mechanism was directly influenced by inhibiting the SGLT1 transport system. (c) 2013 Elsevier Inc. All rights reserved.