Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
Volume 165, Issue 3, Pages 313-318Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpa.2013.03.040
Keywords
trans-Resveratrol; epsilon-Viniferin; Ileum; Jejunum; SGLT1
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trans-Resveratrol and epsilon-viniferin are used as dietary supplements. They are reported to be supportive in preventing arteriosclerosis and diabetes and a previous study could demonstrate an inhibitory potential on sodium-dependent glucose transport (SGLT1) in oocytes und mouse intestinal everted rings (Schulze et al., 2012, Genes Nutr. 6, S61). The in vitro effects of trans-resveratrol and epsilon-viniferin on intestinal glucose uptake in the porcine small intestines (Sus Scrofa) have not yet been evaluated. It was hypothesized that transresveratrol/epsilon-viniferin may have an adverse effect on porcine intestinal sodium-dependent glucose uptake. The effects on electrogenic small intestinal glucose absorption and sodium-dependent H-3-glucose uptake in brush border membrane vesicles (BBMV) were evaluated. Pieces of mucosa were mounted into Ussing chambers and were incubated with either trans-resveratrol (03 mmol/L), epsilon-viniferin (0.3 mmol/L), or ethanol. Sodium-dependent glucose absorption into BBMV was measured. H-3-glucose uptake studies were performed using the same concentrations of the respective substances. SGLT1-mediated glucose absorption was approximately 3-fold higher in ileum compared to jejunum. After preincubation with trans-resveratrol and epsilon-viniferin, glucose-induced increases of short-circuit currents were significantly decreased. BBMV-studies revealed comparable results and glucose uptake was also significantly decreased. As the glucose transport/uptake was decreased after preincubation with either trans-resveratrol or epsilon-viniferin this active transport mechanism was directly influenced by inhibiting the SGLT1 transport system. (c) 2013 Elsevier Inc. All rights reserved.
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